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Cleanliness Assessment Calibration and Reference Slide

DCI’s NIST-Traceable certified calibration and reference slide is aimed at the validation of measurements performed by image analysis instruments for ISO 16232, VDA 19 and and other cleanliness assessment method on 47 mm membrane or paper filters.

The slide was designed to faithfully replicate a typical filter, in color, reflectance, and particles (contaminants) appearance. More than 8000 particles of various sizes are evenly distributed on the slide, allowing a statistically relevant reproduction. For the first time, it is now possible to ascertain that a static image analysis system is actually counting and measuring all particles on a filter.

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Sample report:

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Main Features

Picture
Our 75mm x 50mm x 1mm white media slide has a very similar reflectance to 47mm Paper Filters. This means that using the reference slide will require little to no camera parameters or detection treshold adjustments, reducing the risk of errors due to manipulation.

More than 8 000 particles of various sizes are evenly distributed on the slide, allowing a statistically relevant reproduction.

The particles on our calibration-slide were randomly generated to provide a very wide range of shapes and sizes. This ensures that the detection of every type of particles is validated. Not only have we included “generic” particles, but also the measurements for every single particle detected.

Measurements include:
- Object (Particle) Area,
- Length,
- String Ratio (Fibers Elongation Ratio independently of ''Boxed '' Length and Width),
- Roundness (Based on Circle Equivalent Diameter).
Note: Length only is certified

Not only is the amount of particles on the reference slide ensuring a statiscally significant referal, but we added,
on purpose, some specific objects of interest, along with the with measurements:

Longest fiber (String Ratio › 10:1)
Longest metallic particle (With high reflectance detected at the center)
Longest particle (String Ratio ‹ 10:1)
Longest Round particle (based on equivalent circle area ratio)

Picture
The images of the longest fiber (image shown), longest and largest particle, largest metallic and largest ''round'' particle and their measurements are supplied with the slide.

Measurements

Each certified slide includes two analysis Reports:

• A digital report (on a USB key)
• A printed report.

The Printed analysis report includes:

Mosaic image of full Filter made of 400 camera images.

Two histograms, one for Length distribution as per ISO 16232, and one representing a Linear Length distribution of 10 to 300 microns in 20 bins.

Basic statistics for the 8,000 + particles.

Table of calibration rectangles values.

Image and measurement of Longest Fiber.

The Digital analysis report includes:

Everything found on the printed report plus:

Measurement data of every (8,000+) particles found on the slide, including their individual XY coordinates.

Mosaic Tiff image at full resolution of measured filter scan, made of 400 2.3 Mp Camera images. Individual images of the 400 scanned fields.

Images of the longest fiber, longest particle, largest metallic and largest ''round'' particle, along with their measurements.
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Calibration features

In addition to the particles, the reference slide also includes calibration rectangles that allow calibration of microscope objectives from 50x to 1000x magnification. 5 calibration rectangles are on the slide:

4000, 2000, 1000, 500, 250, 100, 50 μm.
Picture
Blueprint of the calibration slide
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